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rluB [2019-03-19 19:31:49]
ribosomal large subunit pseudouridine synthase B
function
23S rRNA modification
product
pseudouridine synthase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]Gene
Coordinates
2,421,438 2,422,172
The protein
Catalyzed reaction/ biological activity
uridine2605 in 23S rRNA a pseudouridine2605 in 23S rRNA (according to E. coli RluB)Protein family
[SW|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)pseudouridine synthase rsuA family (according to Swiss-Prot)[SW|Domains]
[SW|S4 RNA-binding domain] (aa 2 ... 68) (according to UniProt)Structure
[PDB|4LAB] (from ''E. coli'', 40% identity) [Pubmed|24214967]Expression and Regulation
Operons
genes
[gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]-[gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]-[gene|495721E4B8BF6FEC01E62E86339560F90776EED1|resB]-[gene|97FE84CF968596AC39D19EE2E43DA625147FD702|resC]-[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A106 (ypuL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/106 NBRP B. subtilis, Japan]BKE23160 ([gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA, downstream forward: _UP4_TAAATTTCACATAACCTTCABKK23160 ([gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA, downstream forward: _UP4_TAAATTTCACATAACCTTCAReferences
